Acknowledgement The authors would like to acknowledge Kathryn Moss for her contribution to the HIV monitoring section. Principles of flow cytometric immunophenotyping The primary methods for immunophenotyping in the haematological setting are immunocytochemistry (described in previous editions of this book)…
Acknowledgement The major contribution of the late Dr David Swirsky to this chapter in previous editions of the book is gratefully acknowledged. Erythrocyte cytochemistry Siderocytes and sideroblasts Siderocytes are red cells containing granules of non-haem iron. They were originally described…
The haemoglobin molecule Human haemoglobin is formed from two pairs of globin chains each with a haem group attached. Seven different globin chains are synthesised in normal subjects; two, ε and ζ, are characteristic of the embryo and contribute to…
Assessing the likelihood of acquired haemolytic anaemia Haemolytic anaemia may be suspected from either clinical or laboratory abnormalities. Suggestive clinical features include anaemia, jaundice and splenomegaly. Other relevant clinical features that should be sought are a history of autoimmune disease,…
Appendix Contents Red Blood Cell Testing Estimated Blood Volumes (Plasma Volume and Red Blood Cell Mass) Complete Blood Count (Red Blood Cell) Parameters Red Blood Cell Distribution Width Reticulocyte Count (Absolute and Percentage) Immature Reticulocyte Fraction Reticulocyte Hemoglobin Equivalent Haptoglobin…
Recent progress in chemotherapy and immunotherapy for hematological malignancies has improved cancer survivorship over the past few decades. However, cardiovascular disease is a prominent cause of death in many cancer survivors, with emerging cardiotoxicity from cancer therapies being a significant…
The population is aging, and it is estimated more than 23.4% of the population in the United States will be older than 65 years by 2060. Many hematologic disorders, including anemia and hematologic malignancies such as myelodysplastic disorders, myeloproliferative neoplasms,…
Galen described the spleen as the “organ of mystery,” with functions related to mood and good or ill humors. It was not until the 18th century that the spleen’s relationship to the immune and hematologic systems was appreciated. The complexities…
The management of hematological issues in surgical patients can be challenging due to myriad factors. Bleeding and thrombotic issues may be present concurrently, and the impact of underlying pathological issues may be masked by the primary pathology warranting surgery. Nevertheless,…
Parasitic diseases are not common in medical, let alone hematologic, practice in North America or Europe. However, much of the world’s population is infected by and becomes symptomatic as a result of a plethora of parasites, and many of these…