Acknowledgments My heartfelt appreciation to Ms. Hannah B. Herrera for her reference wrangling. The opinions or assertions contained herein are the private views of the author. Introduction The head and neck, by convention defined as the area above the clavicles…
The impact of diagnostic immunohistochemistry (IHC) for the surgical pathologist is legendary, and it is best appreciated when studying malignancies of unknown primary site. A cost-effective tool, IHC is performed in most hospital laboratories, is often automated, and provides for…
Overview Melanoma continues to represent one of the greatest diagnostic challenges in surgical pathology and is an important source of litigation. Both as a primary lesion in the skin and in metastatic sites, this neoplasm is capable of assuming many…
Only a little more than 30 years ago a popular, widely used classification system for non-Hodgkin lymphomas (NHLs) was initiated based solely on hematoxylin and eosin (H&E)–stained slide interpretation. The “Working Formulation for Clinical Usage” was used for over a…
Overview The hematologic system is complex and includes a diverse range of organs, including the bone marrow, spleen, and lymph nodes. Lymph node disease is covered in Chapter 6 . In this chapter, immunohistochemical evaluation of the bone marrow, spleen,…
Acknowledgment We are grateful to Dr. Mark Wick, who was the author of this chapter in earlier editions and was responsible for much of the content in the sections on biology of antigens and antibodies. Overview Tumors of soft tissue…
Introduction During the last two decades, the approach to histopathologic diagnosis has been dramatically transformed by immunohistochemistry, specifically in the diagnosis and classification of tumors and, more recently, in the diagnosis of infectious diseases in tissue samples. Pathologists play an…
Introduction Quality assurance (QA) in immunohistochemistry (IHC) in the era of precision medicine means that IHC assays are set up to be fit-for-purpose. , Although it is without question that precision medicine depends on accurate diagnosis and classification of various…
Overview Immunohistochemistry (IHC), or immunocytochemistry (ICC), is a laboratory technique used to detect specific antigens (i.e., proteins) in tissues or cells based on antigen-antibody recognition; it seeks to exploit the specificity provided by the binding of an antibody with its…
The practice of surgical pathology has relied traditionally on evaluation of the gross and microscopic features of tissue specimens, integrated with the clinical history and other laboratory data, to reach a definitive diagnosis, assign prognosis, and assist the clinician and…