General Principles of Molecular Biology Immunohistochemistry (IHC) is a common technique used for the detection of protein expression in various tissue samples. In modern pathology practice, this methodology is expanded and complemented by molecular techniques that test for changes in…
Overview Pathologists frequently use immunohistochemistry (IHC) to evaluate antigen location and expression patterns in tissue for diagnostic and/or prognostic purposes. This process often also requires quantification of these immunostains to assist in therapeutic drug selection. Traditional scoring systems for IHC…
Overview Solid neoplasms of childhood and adolescence comprise a diverse group of diagnostically challenging entities with the basic morphologic themes of round cell, spindle cell, and epithelioid tumors. Immunohistochemistry (IHC) and molecular diagnostic tests have greatly improved our ability to…
Acknowledgment The authors thank Dr. Sara Monaco, who was a co-author of this chapter in the previous edition. Immunocytology, or immunocytochemistry (ICC), involves the use of ICC in diagnostic cytopathology. The application of immunostains in cytology cases has expanded and…
Acknowledgments The following colleagues provided particularly valuable assistance. Drs. Noah Brown, Sandra Camelo-Piragua, Katherine McFadden, Jeanne Bell, Larry Junck, and Ricardo Lloyd. Peter Ouillette, Sr BSA provided digital slide preparations, and assistance with Aperio. Linda Cisco carefully scanned slides. We…
Introduction Perhaps the most frequent use of diagnostic immunohistochemistry (IHC) in the surgical pathology laboratory pertains to breast biopsies by virtue of the volume and sheer difficulty of cases. In addition to utilizing IHC for diagnostic problems with breast biopsies,…
This chapter focuses on the use of immunohistochemistry (IHC) in the setting of diagnostic gynecologic pathology. The first section describes the properties and applications of most useful antibodies for the evaluation of lesions in the gynecologic tract. The second section…
Overview The unprecedented advances in cancer genetics and genomics are rapidly affecting the clinical management of solid tumors. Molecular diagnostics are now an integral part of routine clinical management for patients with lung, colon, breast, and gynecologic malignancies among others.…
Overview Used in the proper setting, ancillary techniques can be a great adjunct to light microscopy to obtain an accurate diagnosis in urologic pathology. In the last decade, a plethora of molecular biomarkers have been evaluated for their potential role…
Acknowledgment The authors thank Dr. Hulya Sahin Ozkan for her contributions in the preparation of this chapter. In the ensuing chapter, the cellular lineage markers and the application of immunohistochemistry (IHC) in the diagnosis and management of specific tumor types…