Carboxypeptidase G2

General information

Carboxypeptidases are regulatory peptide-processing enzymes that are secreted by human cells [ ]. Carboxypeptidase G2, which is produced by Pseudomonas strain RS-16, hydrolyses the glutamate residue from methotrexate and other folate analogues [ ] and rapidly hydrolyses methotrexate to the inactive metabolite DAMPA (4-[[2,4-diamino-6-(pteridinyl)methyl]-methylamino]-benzoic acid) and glutamate. It has been used when unexpected toxicity or renal insufficiency occurs during high-dose methotrexate therapy [ ], including intrathecal administration [ ], and for example when folinic acid and other interventions have failed [ , ]. High-dose methotrexate has successfully been used again after rescue with carboxypeptidase G2 [ ].

Drug studies