Introduction ∗ This is an abridged version of this topic in Chapter 6 of the 7 th edition of this text Tissue microarray (TMA) is a method used to evaluate numerous samples of tissue in a short time. First introduced…
Introduction The aim of this appendix is to include discussion and methodology from the chapters Lipids, Proteins, Nucleic Acids and Enzyme Histochemistry which were included in previous editions of this book. These technologies have largely been superseded in the modern…
Key points Whole slide imaging (WSI) allows the creation of digital images of entire histology and cytology slides with sufficient detail to allow viewing at varying magnifications comparable to that achievable with a conventional light microscope. Acquiring digital WSIs requires…
Acknowledgments We thank Richard Davey (SA Pathology) for his assistance in preparing the illustrations for this chapter and Dr Alan Curry (Manchester Royal Infirmary) for his helpful advice on microsporidia. Introduction Transmission electron microscopy (TEM) is a significant tool in…
Acknowledgment The authors acknowledge the use of some of the material and illustrations from the previous edition of this text. Introduction Tumor diagnosis relies centrally on careful morphological examination, supplemented by the judicious use of immunohistochemistry. The information gleaned by…
Introduction The introduction of prognostic and predictive markers in immunohistochemistry (IHC) has made a tremendous beneficial impact on patient diagnosis and management. In the 1940s the demonstration of tissue constituents using an antibody labeled with a fluorescent dye was first…
Acknowledgments Thanks to Sebastian Brandner for providing us with his protocol for methylene blue-azure II-basic fuchsin staining and to Patrick Elliott of the Medical Illustration Department, Royal Hallamshire Hospital, Sheffield, for his illustration depicted in Fig. 18.1 . We are…
Acknowledgments The author would like to thank the following for their contributions to this chapter: Gayle Callis, for tape transfer photographs; and Chris Bull, for his expertise in CT and providing Fig. 17.14 . Introduction Bone is considered the most…
Introduction Microorganisms (or microbes) are organisms which can only be individually seen by microscopy. Many do not cause disease in humans and act as normal colonizers of human hosts. Complex interactions between pathogens, which are capable of causing diseases, the…
Acknowledgments Special thanks to friends, colleagues and patients at the National Amyloidosis Centre for their support and encouragement. Introduction History Amyloidosis is a disorder of protein folding, in which normally soluble proteins misfold and aggregate in a characteristic highly ordered…