KEY POINTS

  • Hematopoietic stem cell transplantation (HSCT) involves the infusion of multipotent hematopoietic stem cells, usually derived from the bone marrow, peripheral blood, or umbilical cord, and using cells from either the patient him-/herself (autologous), a donor (allogeneic), or an identical twin (syngeneic).

  • A preparative or conditioning regimen is a critical element in HSCT for two reasons: to eradicate the underlying disease for which HSCT is given and to provide adequate immunosuppression to prevent rejection of the transplanted graft.

  • Conditioning has traditionally been achieved by delivering maximally tolerated doses of combination chemotherapy with non-overlapping toxicities plus or minus total body radiation; non-myeloablative regimens have been developed as novel approaches to allow older patients or those with comorbid conditions to undergo HSCT.

  • Following HSCT two periods are distinguished: early (within the first 100 days) and late (>100 days); arrhythmias, especially SVT and atrial fibrillation/flutter dominate cardiovascular complications early on, whereas cardiometabolic risk factors, atherosclerotic cardiovascular disease (ASCVD), and heart failure typically present later.

  • The traditional comprehensive history and physical examination by an experienced clinician remains the most critical component of pretransplant screening, supplemented by the HCT comorbidity index.

  • For long-term follow-up, optimal cardiovascular (CV) risk factor control and screening for evolving cardiovascular disease (CVD) is crucial, especially in patients identified as high risk for CVD.

Hematopoietic stem cell transplantation and conditioning regimens

In the past referred to as bone marrow transplantation (BMT) but now termed hematopoietic (stem) cell transplantation (H(S)CT) because of the utilization of stem cell resources other than the bone marrow, HSCT constitutes a life-saving or life-prolonging intervention for many patients with hematologic malignancies and occasionally for patients with benign hematologic and non-hematologic disorders. The indications for HSCT have been rapidly evolving; innovations in HSCT, in combination with new targeted therapies for malignancies, allow older and more medically tenuous patients to be considered for transplantation. These developments have had a significant impact on pretransplant screening and posttransplant monitoring for cardiovascular diseases (CVD) in HSCT recipients.

Two types of HSCT can be distinguished: autologous and allogeneic. Autologous HSCT entails the use of the patient’s own stem cells for reconstitution of the bone marrow. These cells, which are collected in advance, allow for higher dose chemotherapy to be given, which is frequently employed for diseases such as recurrent non-Hodgkin lymphoma, Hodgkin lymphoma, and multiple myeloma. Allogeneic HSCT entails the use of stem cells from a donor to reconstitute the bone marrow. Early studies focused on the use of escalating doses of chemo- and radiation therapy to achieve the dual goals of more effective eradication of chemoresistant malignant cells and preventing immunologic graft rejection by paralyzing the recipient immune system. Chemotherapy doses can be escalated by about three-fold when healthy stem cells from a donor are used after chemotherapy exposure to repopulate the marrow. This strategy is effective because hematopoietic cells are much more sensitive to lethal toxicity compared with other organs, permitting escalation to a dose that is sublethal to other organs, especially the lungs, liver, kidneys, and heart. To permit such high doses of chemotherapy and radiation, patients who were considered appropriate candidates were highly selected to be young (typically <35 years) with excellent organ function.

Subsequent experience clearly indicated that high-dose chemoradiation was only partially responsible for eradication of malignant clone and that immunologic targeting by the donor’s immune system contributed significantly. In fact, this graft-versus-tumor effect was relatively more important in certain diseases, such as chronic lymphocytic leukemia, and relatively less so in other diseases, such as multiple myeloma (MM). It was also associated with potentially severe or life-threatening toxicity when the donor immune system targeted normal recipient tissues and organs resulting in graft-versus-host disease (GVHD) with potentially lethal injury to organs, such as the colon. This can result in bacterial translocation and sepsis, and in the context of immunologic dysregulation a high mortality rate.

Certain diseases that rely predominantly on chemotherapy dose escalation, such as dysproteinemic disorders and lymphomas, typically have much better outcomes when the recipient’s own stem cells are collected in advance of the high-dose conditioning regimen and reinfused afterward (autologous transplantation). This strategy works well when the stem cells are unaffected and the likelihood of transmitting malignant cells with the autologous stem cells is low. The toxicity of this approach is much lower because it avoids immunosuppressive drugs after the stem cell infusion and typically avoids the risk of GVHD. However, for other diseases, such as myeloid disorders, acute leukemias, and bone marrow failure syndromes, a donor-derived (allogeneic) graft is essential for disease control and bone marrow reconstitution, this approach is associated with much greater risk.

Increasing recognition that graft-versus-tumor activity could replace some of the benefit of high-dose chemoradiation for malignant cell eradication led to the possibility of less-intense chemotherapy regimens ( Table 5.1 and Fig. 5.1 ). Progress was accelerated with the development of the purine analogs (e.g., fludarabine and cladribine), which prevent graft rejection with less toxicity. These reduced-intensity or non-myeloablative regimens permitted older patients (into the 7th and 8th decade of life) and more fragile patients to be considered for allogeneic transplantation and thereby the advantage of potentially curative therapy. As a result, the comorbidity and risk spectrum for HCT has increased greatly in the current era.

TABLE 5.1
Preconditioning Regimens for Hematopoietic Cell Transplantation
REGIMEN COMPONENTS CARDIOVASCULAR TOXICITIES
Myeloablative Regimens
Attempts to eliminate all hematopoietic cells in the bone marrow, resulting in profound pancytopenia within 1–3 weeks, which is prolonged, usually irreversible, and often fatal, unless rescued by infusion of hematopoietic stem cells.
BEAM
  • BCNU (carmustine, 300 mg/m 2 ) over 1 day

  • Etoposide (400–800 mg/m 2 ) over 4 days

  • cytosine Arabinoside (800 mg/m 2 ) over 4 days, and Melphalan (140 mg/m 2 ) over 1 day (most common regimen for patients with non-Hodgkin or Hodgkin lymphoma)

  • Carmustine:

    • Chest pain, arterial occlusive disease, tachycardia

    • Etoposide:

    • Hypotension (with rapid infusion) cytosine

  • Arabinoside:

    • Chest pain, pericarditis

  • Melphalan:

    • Atrial fibrillation, peripheral edema

Cy/TBI
  • The Cy/TBI regimen combines cyclophosphamide 120 mg/kg total dose over 2 days

  • Total body irradiation (TBI, 12–13.2 Gy) over 3 days

  • Cyclophosphamide:

    • Arrhythmias

    • Hemorrhagic myocarditis

    • Pericarditis, pericardial effusion, even tamponade

    • Myocardial infarction

    • Arterial and venous thrombosis

    • Radiation-induced heart and vascular disease

TBI/Cy
  • TBI is given first, followed by cyclophosphamide.

  • (may include etoposide (60 mg/kg) instead of cyclophosphamide or in addition to cyclophosphamide for patients with advanced disease not in remission)

Bu4/Cy
  • Busulfan 12.8 mg/kg total dose over 4 days

  • Cyclophosphamide 120 mg/kg over 2 days

  • Busulfan:

    • Arrhythmia, including atrial fibrillation, premature contractions, (complete) atrioventricular block,

    • Peripheral edema

    • Hypertension and hypotension

    • Thrombosis

    • Chest pain

    • Cardiomyopathy (endocardial fibrosis)

Flu/Bu4
  • Fludarabine 120–180 mg/m 2

  • Busulfan 12.8 mg/kg total dose, each over 4 days

  • Fludarabine:

    • Edema

    • Arrhythmia, esp. supraventricular tachycardia

    • Heart failure

    • Angina pectoris

    • Myocardial infarction

    • Cerebrovascular accident

    • Transient ischemic attacks (≤1%)

    • Deep vein thrombosis

    • Phlebitis

    • Aneurysm

High dose melphalan
  • Melphalan (200 mg/m 2 )

  • (common prior to autologous HCT for multiple myeloma; lower dose to be used patient >70 years, with renal dysfunction, or multiple comorbidities.)

See above
Reduced Intensity Regimens
Causes cytopenia, which may be prolonged and can result in significant morbidity and mortality, thus requiring hematopoietic stem cell support.
Flu/Mel
  • Fludarabine (125–150 mg/m 2 total dose) over 5 days

  • Melphalan (140 mg/m 2 ) administered over 2 days

See above
Flu/Bu2 and Flu/Bu3
  • Fludarabine (150–160 mg/m 2 total dose) over 4–5 days

  • Busulfan (8–10 mg/kg) over 2–3 days

See above
Flu/Cy
  • Fludarabine (150–180 mg/m 2 total dose) over 5–6 days

  • Cyclophosphamide (120–140 mg/kg) administered over 2 days

See above
Flu/Bu3/TT
  • Fludarabine 150 mg/m 2 total dose over 3 days

  • Busulfan (8 mg/kg) over 3 days

  • Thiotepa (5–10 mg/m 2 ) over 1–2 days

See above
Nonmyeloablative Regimens
Causes minimal cytopenia (but significant lymphopenia), not requiring stem cell support; however, usually becomes myeloablative because the engrafting donor T cells will eventually eliminate host hematopoietic cells, allowing the establishment of donor hematopoiesis.
Flu/TBI
  • Fludarabine 90 mg/m 2 total dose over 3 days

  • Low dose total body irradiation (TBI, 2 Gy) on the day of graft infusion

See above
TLI/ATG
  • Total lymphoid irradiation (TLI, 8–12 cGy) over 11 days

  • Antithymocyte globulin (ATG; 1.25 mg/kg) over 5 days

  • ATG:

    • Hypertension and hypotension

    • Peripheral edema

    • Tachycardia

    • Chest pain

FIG. 5.1, Illustration of the three main conditioning approaches. The more intense (myeloablative) the protocol, the more toxic it is and typically less reliant on early graft-versus-leukemia (GVL) effect for disease control. Reduced-intensity regimens are less toxic and rely more on an immunotherapeutic GVL effect to prevent relapse. Conditioning may include also the use of antithymocyte globulin in matched unrelated donor (MUD) hematopoietic stem cell transplantation (HSCT). TBI, total body irradiation. *The number represents the radiation dose in rads. † New conditioning in phase II trial for chronic lymphocytic leukemia patients.

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