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Chapter Outline Preparation of commonly used reagents 561 Water 561 Anticoagulants and preservative solutions 561 Buffers 562 Preparation of glassware 564 Cleaning slides 564 Cleaning glassware 564 Sizes of tubes 565 Speed of centrifugation 565 Statistical procedures 565 Calculations 566…
Acknowledgement The major contribution of the late Dr David Swirsky to this chapter in previous editions of the book is gratefully acknowledged. Erythrocyte cytochemistry Siderocytes and sideroblasts Siderocytes are red cells containing granules of non-haem iron. They were originally described…
The haemoglobin molecule Human haemoglobin is formed from two pairs of globin chains each with a haem group attached. Seven different globin chains are synthesised in normal subjects; two, ε and ζ, are characteristic of the embryo and contribute to…
Iron metabolism The iron content of the body and its distribution among the various proteins are summarised in Table 9-1 . Most of the iron is present in the oxygen-carrying protein of the red blood cell, haemoglobin, the synthesis and…
Examination of a fixed and stained blood film is an essential part of a haematological investigation, and it cannot be emphasised too strongly that, to obtain maximum information from the examination, the films must be well spread, well stained and…
Preparation of blood films on slides Blood films should be made on clean glass slides. Films made on coverglasses have negligible advantages and are unsuitable for modern laboratory practice. Films may be spread by hand or by means of an…
It is possible to use manual, semiautomated or automated techniques to determine the various components of the full blood count (FBC). Manual techniques are generally low cost with regard to equipment and reagents but are labour intensive; automated techniques entail…
Eosinophilia is an increase in the number of circulating eosinophils accompanied by an increase of eosinophils and precursors in the bone marrow and often in other tissues as well. The upper limit of normal for the eosinophil count, if subjects…
Although hematopoietic stem cells circulate in small numbers, hematopoiesis, in steady-state conditions in adult life, is largely confined to the bone marrow. All lymphopoietic and hematopoietic cells are ultimately derived from pluripotent hematopoietic stem cells—slowly cycling cells with a capacity…