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Appendix
Chapter Outline Preparation of commonly used reagents 561 Water 561 Anticoagulants and preservative solutions 561 Buffers 562 Preparation of glassware 564 Cleaning slides 564 Cleaning glassware 564 Sizes of tubes 565 Speed of centrifugation 565 Statistical procedures 565 Calculations 566…
Haematology in Under-Resourced Laboratories
Introduction: types of laboratories In most countries, there are likely to be some laboratories with limited resources, but in under-resourced countries, the majority of laboratories face chronic shortages of trained staff, low morale, inadequate and poorly maintained equipment and erratic…
Quality Assurance
Quality assurance (QA) in the haematology laboratory is intended to ensure reliable diagnostic test results with the necessary degree of accuracy and precision. There are some key definitions in QA that are fundamental to an understanding of its practice (…
Laboratory Organisation, Management and Safety
Acknowledgement The authors wish to acknowledge the major contribution of Dr Mitchell Lewis, the author or co-author of this chapter in previous editions. The services provided by laboratories are an essential and fundamental component of health systems across the globe.…
Approach to the Diagnosis and Classification of Blood Cell Disorders
Common presentations of haematological diseases An abnormal blood count or blood cell morphology does not necessarily indicate a primary haematological problem because it may reflect an underlying nonhaematological condition or may be the result of therapeutic interventions. Anaemia occurs in…
Laboratory Aspects of Blood Transfusion
Safe and effective blood transfusion requires the combined efforts of blood transfusion services, biomedical scientists and clinicians to ensure the highest standards are applied to all the systems in a complex process from ‘vein to vein’. This chapter provides a…
Blood Cell Antigens and Antibodies: Erythrocytes, Platelets and Neutrophils
Erythrocytes Red cell antigens Since Landsteiner’s discovery in 1901, that human blood groups existed, a vast body of serological, genetic and biochemical data on red cell (blood group) antigens has been accumulated. More recently, the biological functions of some of…
Laboratory Control of Anticoagulant, Thrombolytic and Antiplatelet Therapy
Anticoagulant and antithrombotic therapy is given to prevent formation or propagation of thrombi. Anticoagulant drugs, unlike fibrinolytic agents, have little if any effect on an already-formed thrombus. There are five main classes of drugs that require consideration: 1. Coumarins and…
Investigation of a Thrombotic Tendency
Introduction to thrombophilia Investigations to identify an acquired or inherited thrombotic tendency are most frequently carried out in patients who develop venous or arterial thrombosis at a young age, in those who have a strong family history of such events…