Non-Neoplastic and Tumor-Like Conditions of the Ovary

Definitions

Dysfunctional ovarian cysts derive from the follicular apparatus either before or after ovulation ( Table 24.1 ). They may result from or cause disordered hypothalamic–pituitary–ovarian function. Although not always functional in the sense of producing steroid hormones, the cysts all have or have had the potential to do so at some stage in their development.

Ultrasound examination is used widely in the diagnosis and surveillance of these cysts, which achieve clinical importance if they exceed 3 cm in premenopausal and early postmenopausal women or 1 cm in late postmenopausal women, and if they do not disappear on repeat scanning. Most disappear spontaneously within 2–3 weeks. Cyst aspiration is not recommended, as the cysts often recur and the sensitivity for the detection of malignancy is low.

‘Polycystic ovaries’ is a term that should be reserved for the abnormal ovaries found in association with functional hyperandrogenism (the Stein–Leventhal and related clinical syndromes; see later). The ovaries commonly have thick white capsules, and display multiple cystic follicles and small, luteinized follicular cysts of atretic type, absence of stigmata of recent ovulation, and occur with a characteristic disturbance of hypothalamic–pituitary function. Ovaries displaying several cysts, but otherwise not fitting into this category, should be called ‘multicystic’ to avoid confusion.

Corpus luteum cysts also derive from the follicular apparatus but show evidence of prior ovulation, which distinguishes them from follicular cysts. Ovulation is indicated by convolution of the cyst lining (which results from collapse of the follicle) with the characteristic invaginations of theca–lutein (paralutein) cells into the inner zone of granulosa lutein cells. A layer of fibrous tissue lining the inner surface of the cyst, resulting from organization of the corpus luteum hematoma, also indicates that ovulation has occurred.

Features distinguishing the various types of cysts derived from the follicular apparatus appear diagrammatically in Figure 24.1 .

Etiology

The etiology of follicular cysts (by definition ≥3.0 cm) is not always obvious but in most cases reflects disordered function of the pituitary–ovarian axis. Pathologic cystic change may develop either in the follicular growth phase or during atresia. Unluteinized follicular cysts ( Figure 24.1A ), which produce predominantly estradiol, result from excessive ovarian stimulation (either by endogenous follicle-stimulating hormone (FSH) or by ovulation-induction agents) or an abnormal response to normal stimulation.

Granulosa lutein cysts are follicular cysts with predominantly luteinized granulosa cells (which are internal to the basal lamina; see Figure 24.1B ). Like corpora lutea, the granulosa cells secrete progesterone. An unknown proportion of granulosa lutein cysts results from failure of follicular rupture at the expected time of ovulation and is the basis of the luteinized unruptured follicle syndrome, which occurs more frequently in infertile than in normal fertile women.

Theca–lutein cysts are follicular cysts with luteinization predominantly of the theca interna (which is external to the basal lamina; see Figure 24.1C ). Androstenedione is the characteristic steroid product. These cysts develop when there is prolonged exposure to luteinizing hormone (LH) or beta human chorionic gonadotropin (β-hCG), either endogenous, such as in polycystic ovary syndrome (PCOS; see later) or hyperreactio luteinalis syndrome of pregnancy (multiple theca–lutein cysts), or exogenous, as in the ovarian hyperstimulation syndrome. Atretic follicular cysts can also elaborate androstenedione.

Clinical Features

Ovarian cysts in the fetus are more frequent than were once thought, with an estimated incidence of about one in 2500 births. They are usually diagnosed in the third trimester by ultrasound or magnetic resonance imaging (MRI). A cyst size below 4–5 cm usually carries a low risk of torsion, whereas larger cysts have a high risk, and therefore may warrant decompression. However, the criteria and recommendations for therapy vary widely. Since torsion can lead to ovarian loss and thus impact future fertility, the goal of intervention is to preserve fertility. Therapeutic intervention is usually prompted by acute torsion in the newborn or persistent cysts exceeding 5 cm in diameter in children older than 6 months of age.

In prepubertal girls, follicular cysts form a significant proportion of ovarian lesions that come to surgical intervention. Common clinical presentations are pain, vomiting, diarrhea, and constipation, sometimes precipitated by ovarian torsion. A less common but well-recognized association is with isosexual pseudoprecocity, either idiopathic or associated with hypothyroidism or the McCune–Albright syndrome (polyostotic fibrous dysplasia, cutaneous melanin pigmentation, and endocrine organ hyperactivity).

During the reproductive years, unluteinized follicular cysts may produce sufficient estradiol to cause irregular or prolonged (dysfunctional) uterine bleeding. The endometrium in such cases may show disordered proliferative phase changes or hyperplasia. Granulosa lutein cysts secrete progesterone, but rarely in sufficient amounts to disturb the menstrual cycle. Atretic cysts, if numerous (as in the PCOS), may synthesize sufficient androstenedione to produce hirsutism or virilization. Observation shows that most regress spontaneously over a few cycles and that therapy with contraceptive agents does not accelerate this process. Persistence should raise the suspicion of neoplasia.

During pregnancy, pre-existing follicular cysts may become luteinized. New cysts rarely develop but a notable exception is the hyperreactio luteinalis syndrome (multiple theca–lutein cysts). Large solitary luteinized follicular cysts may present as adnexal masses in the third to fourth months of pregnancy or postpartum, or are incidental findings at cesarean section.

Symptomatic follicular cysts are rare after menopause, although some sporadic follicular activity (generally without ovulation) continues in the early years.

Gross Features

These are usually single and 3–10 cm in diameter. Solitary luteinized follicle cysts of pregnancy and puerperium have a median diameter of 25 cm. They are smooth lined and contain clear proteinaceous fluid or altered blood.

Microscopic Features

Unluteinized follicular cysts show the microscopic features of physiologic growing cystic follicles, with well-preserved granulosa and theca layers separated by a basal lamina ( Figure 24.2 ). However the cumulus oophorus and oocyte are no longer present. The lining may be somewhat attenuated secondary to dilatation. There is no trace of the festooned pattern of a corpus luteum. During involution the lining is often incomplete and later appears as small clusters of lutein cells in the compressed ovarian stroma, which becomes progressively fibrotic. As involution continues, distinguishing features become increasingly difficult to identify and definitive diagnosis may not be possible ( Figure 24.3 ); in this situation the cyst is better designated ‘simple’ (see later).

Cytologically, unluteinized follicular cysts contain numerous granulosa cells that have been shed into the fluid and reflect the normal lining. The cells are arranged singly and in tight clusters. The clusters may be irregular in shape, some with papillary configurations. Granulosa cells contain round to oval nuclei and a small rim of distinct cytoplasm. The chromatin is coarsely granular. Nuclear grooves are occasionally seen. Mitoses may be identified. The cells are uniform with negligible variation from cell to cell. Degenerative changes may be present.

Luteinized follicular cysts show the general features of unluteinized follicular cysts as described above, but in addition some of the cells in the cyst wall are luteinized. The granulosa (granulosa lutein cyst; see Figure 24.4 ) or theca layer (theca–lutein cyst; see Figures 24.5 and 24.6 ), or occasionally both, luteinize, although the cyst is classified according to the layer predominantly affected. Luteinization appears as enlarged cells with increased eosinophilic or finely vacuolated cytoplasm. The changes are similar to those in a corpus luteum.

Cytologically, luteinized follicular cysts contain single and/or clusters of luteinized granulosa cells with round to oval nuclei, coarse chromatin, and small prominent nucleoli. Cell cytoplasm is ample and foamy. Mitotic figures may be found. The cells lack pleomorphism. Degenerative changes may be present in single cells with obvious nuclear pyknosis.

Atretic follicular cysts are lined by ovarian stroma in which there are small clusters of luteinized theca cells. They may be indistinguishable from involuting follicular cysts of other types.

Differential Diagnosis

Cysts derived from a corpus luteum can be distinguished from luteinized follicular cysts by their retention, partially or focally, of their convoluted lining with invaginations of paralutein cells, and by their innermost lining of fibrous tissue (the result of organization of the central coagulum). In addition a well-defined vascular zone may be evident.

Cystic granulosa cell tumors may be difficult to distinguish from follicular cysts. They are often considerably larger and always unilateral. The neoplastic cells lining the cysts may show prominent luteinization and may display mitotic activity. Although most granulosa cell tumors are estrogenic, 15–30% of the cystic variants are clinically androgenic, a feature not associated with follicular cysts except in the specific clinical situations mentioned above.

Etiology

These are cysts derived from corpora lutea of menstruation or pregnancy that result from excessive central hemorrhage. Small physiologic hemorrhages occur 2–4 days after ovulation during the stage when the corpus luteum vascularizes. They usually organize rapidly, but if the hemorrhage is excessive and the corpus luteum becomes overdistended with blood, involution is likely to be delayed.

Clinical Features

Most are asymptomatic. In some non-pregnant women, this delayed involution (sometimes called ‘persistent corpus luteum’) and its continuing production of progesterone may result in minor menstrual disturbances (so-called ‘irregular shedding’).

Gross Features

Corpora lutea of menstruation rarely exceed 3 cm (average size 2 cm) but may do so if there is an unusually large fluid-filled central cavity. Cystic corpora lutea of pregnancy often exceed 5 cm and may exceed 10 cm in diameter. They are not infrequently identified on routine ultrasound examination in early pregnancy. They contain clear fluid or altered blood. Rupture may be evident. They are generally smooth lined with an incomplete band of yellow tissue in the wall.

Microscopic Features

Cystic corpora lutea display the same features as their non-cystic counterparts. The distension leads to some attenuation of the convolutions but without loss of distinctive cell layers. The fibrous tissue lining contains mature collagen demonstrable with a trichrome connective tissue stain. Peripheral clusters of small paralutein (theca–lutein) cells may still be seen between larger groups of granulosa lutein cells ( Figure 24.7 ). The granulosa and theca–lutein cells are smaller than those seen in a fresh corpus luteum. The nuclei are small, hyperchromatic, and lack mitotic activity, while the cytoplasm is usually finely vesicular. With progressive involution, fewer and smaller islands of lutein cells are found within the increasingly fibrotic wall, but a well-defined zone of small blood vessels remains to mark the phase of vascularization ( Figure 24.8 ).

Cytologically, corpus luteum cysts contain numerous fully luteinized granulosa cells singly and in clusters and mixed with fresh blood and scattered hemosiderin-laden macrophages. Unlike those from follicular cysts, these cells are large and polyhedral in shape with eccentric, round nuclei. Hemorrhagic corpus luteum cysts may be identified by these features as well as the presence of abundant fibrin and some fibroblasts in the smear background. Regressing corpora lutea may contain large luteinized cells, as well as numerous macrophages with yellow hematoidin pigment.

Differential Diagnosis

Luteinized follicular cysts appear macroscopically like corpus luteum cysts but are less likely to be hemorrhagic. The microscopic distinction hinges around evidence of ovulation as indicated earlier. Follicular cysts generally lack the zone of vascularization. Some long-standing cysts may be very difficult to classify.

Endometriotic cysts may be impossible to distinguish macroscopically from corpus luteum cysts but should be suspected if there is evidence of endometriosis elsewhere. Microscopic identification of endometrial glands or stroma within the cyst confirms the diagnosis.

Definition

These are ovarian cysts or cyst-like structures that lack identifiable linings. Some produce symptomatic adnexal masses but most are incidental findings. The cysts are usually less than 10 cm in diameter, solitary, and have a smooth internal surface and a variable content of clear fluid or altered blood. Most are of follicular origin, although some arise as epithelial cysts.

Microscopic Features

The lining usually consists only of a narrow band of dense fibrous tissue or compressed ovarian stroma. There may be occasional small clusters of involuting lutein cells in the cyst wall, suggesting an origin from the follicular apparatus. Sometimes there is a complete or partial lining of apparent epithelial cells that are too attenuated for their origin to be identified ( Figure 24.9A ) but, even here, persistent reactivity for α-inhibin may be present ( Figure 24.9B ). Some cysts undoubtedly represent epithelial cystomas. Some are lined by granulation tissue or organizing hematoma. Such lesions may be endometriotic in origin but lack overall the necessary diagnostic features. There is probably no benefit in precisely categorizing ‘simple’ cysts. All are benign and most are non-neoplastic.

Definition

These are multinodular, often multicentric or bilateral, tumor-like masses of lutein cells that develop during an otherwise normal pregnancy (presenting usually in the third trimester) and involute in the puerperium. They represent the extreme example of nodular theca–lutein hyperplasia of pregnancy.

Clinical Features

About one-fourth of patients become hirsute or virilized. Approximately 80% of cases occur in black women. Rarely, female fetuses display masculinizing signs at birth.

Etiology

The lesions most likely arise from the lutein cells found during pregnancy as isolated groups in the stroma or in the walls of atretic follicles. Some patients have a history suggestive of PCOS. β-hCG is necessary for the development of luteomas, but is unlikely to be the only etiologic factor since the lesions are not associated with trophoblastic disease or with early pregnancy, when β-hCG levels are at their highest. Morphologic and clinical similarities exist between luteomas and multiple theca–lutein cysts (hyperreactio luteinalis), and it is possible that they are related forms of theca–lutein cell hyperplasia. Unlike luteomas, hyperreactio luteinalis is associated with conditions of pathologic β-hCG elevation, multiple gestations, and abnormal pregnancy states.

Gross Features

Luteomas are usually 6–12cm in diameter but may exceed 20 cm. They are circumscribed, multinodular solid masses with a soft gray-brown to tan cut surface. Small cystic spaces may be present. Those removed in the puerperium may be softer and paler or even frankly necrotic.

Microscopic Features

Luteomas display sheets of large eosinophilic cells that are broken up into groups by numerous delicate blood vessels. An organoid pattern ( Figure 24.10 ) is common. A trabecular arrangement is less frequently seen. The cell size is intermediate between that of granulosa and theca–lutein cells. The reticulin pattern of fibers around groups rather than individual cells is also intermediate between the abundant pericellular distribution of reticulin fibers in the theca interna and the paucity of fibers in the granulosa layer. Follicular spaces containing colloid-like material may be present but the intracellular hyaline droplets (‘colloid bodies’) typical of corpora lutea of pregnancy are rare. The cytoplasm is eosinophilic and sometimes finely vacuolated but contains little stainable lipid. The round central nuclei have a prominent nucleolus and abundant euchromatin. There may be mild nuclear pleomorphism and some mitotic figures (<3 per 10 HPF) ( Figure 24.10 ). The remainder of the ovary shows the physiologic changes of pregnancy but small theca–lutein cysts may be evident as well. Early involutional changes include nuclear pyknosis and increased cytoplasmic vacuolization. Eventually the luteoma is reduced to sheets of necrotic cells ( Figure 24.11 ). Even at this late stage, the typical reticulin pattern may still be useful diagnostically.

Differential Diagnosis

Non-cystic aggregates of large lutein-like cells include a plethora of entities in the differential diagnosis, some neoplastic, some not ( Figure 24.12 ). The clinical context and gross features of the lesions all contribute to their separation.

Nodular stromal hyperthecosis may occur in pregnancy. The ovaries are uniformly enlarged and display multiple microscopic nodules of spindle-shaped luteinized stromal cells, arranged in bundles rather than nests. Reticulin fibers invest individual cells that have pale or finely vacuolated cytoplasm containing abundant lipid.

Steroid cell tumors are almost always unilateral (excepting for stromal luteomas) and sometimes arise in the ovarian hilus. They are solid, lobulated tumors composed of eosinophilic or finely vacuolated cells arranged in a diffuse or, less commonly, a trabecular pattern—not dissimilar to that of luteomas. Reticulin surrounds single cells or small groups. Mitotic activity is variable but rarely as prominent as in luteomas of pregnancy. The most helpful distinguishing microscopic features are the abundant stainable intra­cytoplasmic lipids in all steroid cell tumors and the presence of crystalloids of Reinke in Leydig cell tumors ( Table 24.2 ).

Thecomas are usually unilateral. They may be focally luteinized in pregnancy and resemble luteomas. However, the basic spindle shape of the cells is still evident. Sclerosing stromal tumors, closely related to thecomas, display a striking lobulated pattern that is due to bands of edematous immature or mature collagenous stroma (absent in luteomas). Within the lobules is abundant pericellular reticulin as seen in thecomas. Another helpful distinguishing feature is the mixed population of spindle and polyhedral eosinophilic cells (mostly the latter). Granulosa cell tumors are rare in pregnancy but, when they occur, are usually of the juvenile type, which may closely resemble luteomas.

Etiology and Clinical Features

While theca–lutein cysts (luteinized follicular cysts) occur at any age and in many different clinical situations, multiple bilateral theca–lutein cysts are classically associated with molar pregnancies or choriocarcinoma, occurring in 25% of such cases. They also occur with Rh isoimmunization, nonimmune hydrops, chronic renal failure, multiple pregnancies, and even apparently normal singleton pregnancies. Rarely, a similar clinicopathologic picture results from ovarian hyperstimulation, provoked by ovulation-induction agents (see later), a condition occasionally associated with clinical evidence of hyperglycemia or virilization. Although multiple bilateral theca–lutein cysts are usually associated with abnormally elevated β-hCG levels, additional factors may be necessary for their genesis. The cysts may persist into, or appear first, in the puerperium when β-hCG levels have fallen. In the latter situation the cysts probably initially developed during pregnancy under the influence of β-hCG but were then maintained by the FSH and LH levels that rose soon after parturition if lactation was not established. The condition almost always regresses within a few weeks after parturition. For this reason surgery during pregnancy, which is often required for diagnostic purposes or management of acute abdomen or shock, should be as conservative as possible. Intraoperative frozen-section examination of an incisional ovarian biopsy may obviate unnecessarily extensive surgery based on the erroneous presumption of malignant disease.

Gross and Microscopic Features

Both ovaries are involved and measure up to 15 cm across. Sectioning shows multiple cysts 1–4 cm in diameter that contain yellowish fluid or blood, separated by edematous stroma ( Figure 24.13 ). The follicular cysts show hyperplasia and prominent luteinization of the theca interna layer; the granulosa is often luteinized as well ( Figures 24.6 and 24.14 ). The edematous stroma may also contain large clusters of luteinized stromal cells.

Differential Diagnosis

Solitary luteinized follicular cysts of pregnancy and the puerperium (see later) are large, unilateral, and unilocular. Hemorrhage and edema are usually absent. There is no distinct separation of granulosa and theca layers. Cystic ovarian neoplasms, while also in the differential diagnosis, can be easily distinguished from hyperreactio luteinalis by microscopic examination.

Definition

A large, distinctive follicular cyst of the ovary may occur during pregnancy. Such cysts present as adnexal masses in the third to fourth months of pregnancy or postpartum, or are incidental findings at cesarean section. The involved ovaries exhibit large (average diameter 25 cm), unilocular, thin-walled cysts ( Figure 24.15 ), which contain clear or mucoid fluid. The pathogenesis is unknown, but β-hCG stimulation is probably important.

Microscopic Features

Microscopically, a single layer or multiple layers of large luteinized cells line the cysts, with only a sparse reticulin network. Similar cells are sometimes also present in the fibrous wall of the cysts but these cells are not obviously thecal in type, i.e., there is no clear definition of the granulosa and theca elements of the cyst wall usually seen in cysts of follicular origin. A striking feature is the focal presence of large, pleomorphic and hyperchromatic nuclei in the luteinized cells ( Figure 24.16 ). This feature, together with the remarkably large cyst size and lack of recognizable separation of lining cells into granulosa and theca layers, distinguish this entity morphologically from the follicular cysts of non-pregnant women. Mitotic figures are lacking.

Etiology

Deciduosis (extrauterine decidual change or ectopic decidua) is a quasi-physiologic process that arises in the subcelomic mesenchyme as a result of the progesterone stimulus of pregnancy. It is thus an expression of müllerian­osis. It can be identified, if carefully sought, in the cortex or surface of most ovaries from term or near-term pregnancies. It is found with greater difficulty in the first and second trimesters. Deciduosis also develops on the peritoneal surfaces of other pelvic and abdominal structures and may be exaggerated in patients with trophoblastic disease. This decidual change is also regularly observed in the stroma of ovarian endometriotic deposits during pregnancy.

Gross and Microscopic Features

Deciduosis appears as serosal macules 1–5 mm across, which are flat or slightly raised in contour. Decidual foci consist of superficial discrete collections of cells cytologically similar to the decidual cells of gestational endometrium, i.e., distinct cell margins, abundant, slightly eosinophilic, finely granular cytoplasm, and central, small, round pale nuclei with conspicuous nucleoli. Capillaries are prominent and the decidual cells sometimes appear to sheathe them. A sprinkling of lymphocytes may be present ( Figures 24.17 and 24.18 ). Most commonly the foci are nodular or plaque-like but some lie just beneath the serosal surface and are surrounded by edematous stroma. Decidual foci may become confluent.

Definition

Proliferations of granulosa cells occur rarely as incidental findings in pregnant women. The lesions are usually multiple and are present within atretic follicles.

Microscopic Features

The arrangement of the granulosa cells mimics similar patterns seen with granulosa cell tumors, i.e., solid, microfollicular, trabecular, or insular ( Figure 24.19 ). Usually, the granulosa cells contain scanty cytoplasm and grooved nuclei resembling the cells of the adult-type granulosa cell tumor. Less commonly, the cells are luteinized with non-grooved nuclei of variable size, or sertoliform with vacuolated cytoplasm suggestive of lipid.